发明名称 |
GENERATION AND USE OF FAB, SCFV, AND RELATED BINDING MOLECULES SPECIFIC FOR HIV-1 REV |
摘要 |
Described herein is the identification, though phage display, of a chimeric rabbit/human anti-Rev Fab (SJS-R1) that readily solubilized polymeric HIV-1 Rev. The Fab binds with very high affinity to a conformational epitope in the N-terminal half of HIV-1 Rev. The corresponding single chain antibody (scFv) was also prepared and characterized. Methods of making and using SJS-R1 Fab and SJS-R1 scFv, and antibodies and antibody fragments that share at least one CDR with SJS-R1 Fab, are provided. Specific described methods include methods of preventing or reversing polymerization of HIV Rev, methods of preventing or inhibiting replication of a lentivirus in a cell, methods of reducing infectivity of replication of a lentivirus, inhibiting Rev function in a cell infected with a lentivirus, and methods of treating a disease or symptom associated with Rev expression in an animal. |
申请公布号 |
US2012201811(A1) |
申请公布日期 |
2012.08.09 |
申请号 |
US201213366015 |
申请日期 |
2012.02.03 |
申请人 |
STAHL STEPHEN J.;WATTS NORMAN R.;WINGFIELD PAUL T.;RADER CHRISTOPH;STEVEN ALASDAIR C.;SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES;THE UNITED STATES OF AMERICA AS REPRESENTED BY THE |
发明人 |
STAHL STEPHEN J.;WATTS NORMAN R.;WINGFIELD PAUL T.;RADER CHRISTOPH;STEVEN ALASDAIR C. |
分类号 |
A61K39/395;A61P31/18;C07K2/00;C07K16/10;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N7/06;C12N9/96;C12N15/13;C12N15/63 |
主分类号 |
A61K39/395 |
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