| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a method for identifying a target for the development of a therapeutic drug for responding to disease conditions. <P>SOLUTION: A method for identifying host genes and encoded proteins for potential targets for therapeutic intervention employs a Gene Search Vector that is either lentivirus or MMLV-based, and can be used to interrogate an entire cell genome without prior knowledge of a genomic sequence. This Random Homozygous Gene Perturbation (RUGP) technique is used to identify potential host targets for intervention for influenza, HIV and other viral infections. Using Thermal Assymetric Interlaced (TAIL)-PCR, the period for identification of promising targets is reduced from months to weeks or less. Specific substances including PTCH1, Robo1 and Nedd4 are targeted. <P>COPYRIGHT: (C)2012,JPO&INPIT |
