发明名称 |
HIGH THROUGHPUT DETECTION OF MOLECULAR MARKERS BASED ON AFLP AND HIGH THROUGH-PUT SEQUENCING |
摘要 |
The present invention relates to a high throughput method for the identification and detection of molecular markers wherein restriction fragments are generated and suitable adaptors comprising (sample-specific) identifiers are ligated. The adapter-ligated restriction fragments may be selectively amplified with adaptor compatible primers carrying selective nucleotides at their 3′ end. The amplified adapter-ligated restriction fragments are, at least partly, sequenced using high throughput sequencing methods and the sequence parts of the restriction fragments together with the sample-specific identifiers serve as molecular marker. |
申请公布号 |
US2012202698(A1) |
申请公布日期 |
2012.08.09 |
申请号 |
US201213449629 |
申请日期 |
2012.04.18 |
申请人 |
VAN EIJK MICHAEL JOSEPHUS THERESIA;HOGERS RENE COMELIS JOSEPHUS;KEYGENE N.V. |
发明人 |
VAN EIJK MICHAEL JOSEPHUS THERESIA;HOGERS RENE COMELIS JOSEPHUS |
分类号 |
C40B20/00;C07H21/04 |
主分类号 |
C40B20/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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