| 摘要 |
<p>The present invention addresses the problem of providing: a method for culturing human pluripotent stem cells still remaining in an undifferentiated state, and a kit to be used with the method, the method being more efficient than conventional methods. It has been confirmed that the pluripotency of stem cells is efficiently maintained, irrespective of how proficient an experimenter is in regard to culturing techniques, in a case where: (a) human pluripotent stem cells are cultured in a first medium, which is a pluripotent stem cell medium including activin; (b) the first culture is replaced with a second culture, which is an activin-free pluripotent stem cell medium, and the human pluripotent stem cells are cultured; (c) the human pluripotent stem cells are sub-cultured in the first medium; and thereafter (b) and (c) are repeated in the stated order.</p> |
