摘要 |
<P>PROBLEM TO BE SOLVED: To provide a method for extracting an antigen of multidrug resistant staphylococcus capable of detecting the antigen with higher layer efficiency than that of alkali aqueous solution, and capable of also detecting each toxin, and to provide a method for detecting protein obtained by being extracted as the antigen by an immune reaction. <P>SOLUTION: An antigen of staphylococcus is extracted under an acid condition. It is preferable to perform extraction under existence of surfactant. It is preferable to perform the extraction for 15 seconds to 60 minutes under conditions of pH3 or less at a temperature from room temperature to a boiling point condition. According to the present invention, a toxin of the staphylococcus, or protein (PBP2') which rules β lactam drug resistance is promptly, simply, and efficiently extracted. In addition, the toxin is detected. According to the present invention, the extracted toxin and the protein containing PBP2' are utilized for immune measurement utilizing an antigen-antibody reaction as the antigen. <P>COPYRIGHT: (C)2012,JPO&INPIT |