| 摘要 |
[Problem] To produce functional liver cells, which are usable in testing the metabolism and toxicity of a drug, from pluripotent stem cells. [Solution] A method for producing highly functional liver cells using pluripotent stem cells, said method being characterized by comprising, from the pluripotent stem cells, acquiring primitive endoderm derived from the pluripotent stem cells by a process involving steps (A) and (B), from the primitive endoderm derived from the pluripotent stem cells, acquiring liver precursor cells by a process involving step (C), and, from the liver precursor cells, acquiring the highly functional liver cells by a proecss involving step (D): (A) a step for culturing under serum-free and feeder-free conditions; (B) a step for culturing in the presence of albumin and at least one kind of cytokine; (C) a step for culturing in the presence of SHH or an SHH agonist and at least one kind of cytokine; and (D) a step for culturing and maturing in the presence of at least one kind of cytokine. |
