Method for producing a target protein connected with a heterogeneous ferritin protein using yeast host cells

摘要

Producing a target protein, comprises (a) providing a cell culture with host cells of a yeast species e.g. Saccharomyces, (b) disposing the host cells with the aid of the first DNA sequence encoding for the heterologous protein-ferritin and the second DNA sequence encoding for the target protein to express the ferritin protein and the target protein containing composite protein, where the composite protein is maintained in the host cells, (c) separating the composite protein from the cell culture, and (d) separating the target protein from the composite protein. Producing a target protein, comprises (a) providing a cell culture with host cells of a yeast species Saccharomyces, Schizosaccharomyces, Kluyveromyces, Hansenula, Arxula, Schwanniomyces, Candida, Pichiaor Yarrowia, where the host cells contain at least a first DNA sequence encoding a heterologous protein-ferritin and also contain at least one second DNA sequence encoding for the target protein; the second DNA sequence is coupled with the first DNA sequence in such a way that in the expression of the first and the second DNA sequence, the target protein is coupled to the previously synthesized ferritin protein such that a composite protein is formed, (b) disposing the host cells with the aid of the first DNA sequence encoding for the heterologous protein-ferritin and the second DNA sequence encoding for the target protein to express the ferritin protein and the target protein containing composite protein, where the composite protein is maintained in the host cells, (c) separating the composite protein from the cell culture, and (d) separating the target protein from the composite protein. An independent claim is included for the host cell of the yeast species.