发明名称 |
DEVELOPMENT OF GRAIN WEEVILS RESISTIVE MOLECULAR MARKERS IN MUNG BEANS AND USES THEREOF |
摘要 |
<p>PURPOSE: A molecular marker for determining Callosobruchus chinensis-resistant mung beans is provided to improve determination efficiency and to shorten generation interval. CONSTITUTION: A primer pair for determining Callosobruchus chinensis-resistant mung beans contains two bases selected from the group consisting of sequence numbers 1-17. A method for detecting Callosobruchus chinensis-resistant plants comprises a step of analyzing genome DNA of a plant under the presence of the primer pair by RFLP(restriction fragment length polymorphism), RAPD(randomly amplified polymorphic DNA), DAF(DNA amplification fingerprinting), AP-PCR(arbitrarily primed PCR), STS(sequence tagged site), EST(expressed sequence tag), SCAR(sequence characterized amplified regions), ISSR(intersimple sequence repeat amplication), AFLP(amplified fragment length polymorphism), CAPS(cleaved amplified polymorphic sequence) or PCR-SSCP(single-strand conformation polymorphism). A kit for determining Callosobruchus chinensis-resistant mung beans contains the primer pair.</p> |
申请公布号 |
KR20120088400(A) |
申请公布日期 |
2012.08.08 |
申请号 |
KR20110009732 |
申请日期 |
2011.01.31 |
申请人 |
REPUBLIC OF KOREA(MANAGEMENT:RURAL DEVELOPMENT ADMINISTRATION) |
发明人 |
KIM, YUL HO;PARK, HYANG MI;CHOI, MAN SOO;JUNG, JIN KYO;MOON, JUN KYUNG;SEO, MIN JUNG;LEE, SEUK KI;YUN, HONG TAI;KIM, WOOK HWAN |
分类号 |
C12N15/11;C12Q1/68 |
主分类号 |
C12N15/11 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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