METHOD FOR PREPARING A CARBOXYLIC ACID USING NITRILASE ORN

摘要

PURPOSE: A novel nitrilase ORN and a method for preparing carboxylic acid using the same are provided to ensure substrate specificity and to be used as a material for pharmaceutical product synthesis. CONSTITUTION: A method for preparing carboxylic acid comprises: a step of preparing nitrilase ORN or a biocatalyst composition containing nitrilase ORN; and a step of reacting a nitrile substrate with nitrilase ORN or the biocatalyst composition. The nitrile substrate includes acetonitrile, acrylonitrile, adiponitrile, benzonitrile, butyronitrile, 4-chlorobenzonitrile, or mandelonitrile. Nitrilase ORN is prepared by a transformant. The transformant is prepared by transforming with a vector containing an nucleic acid molecule encoding an amino acid of sequence number 5.