发明名称 |
METHOD FOR NUCLEOTIDE DETECTION |
摘要 |
A method of inhibiting light-induced degradation of nucleic acids includes irradiating a portion of the nucleic acids in the presence of a detection solution comprising a polyphenolic compound. A method of detecting a nucleic acid having a fluorescent tag includes irradiating at least a portion of the nucleic acid with light of a suitable wavelength to induce a fluorescence emission and detecting the fluorescence emission. Optionally, the polyphenolic compound is gallic acid, a lower alkyl ester thereof, or mixtures thereof. A kit includes one or more nucleotides, an enzyme capable of catalyzing incorporation of the nucleotides into a nucleic acid strand and a polyphenolic compound suitable for preparing a detection solution.
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申请公布号 |
US2012196758(A1) |
申请公布日期 |
2012.08.02 |
申请号 |
US201113018255 |
申请日期 |
2011.01.31 |
申请人 |
KLAUSING KAY;SMITH VINCENT;SHEN MIN-JUI RICHARD;MOORE JOHN;HALL KEVIN |
发明人 |
KLAUSING KAY;SMITH VINCENT;SHEN MIN-JUI RICHARD;MOORE JOHN;HALL KEVIN |
分类号 |
C40B20/04;C12Q1/68;C40B30/00 |
主分类号 |
C40B20/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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