| 摘要 |
An object of the present invention is to provide novel ribozyme systems capable of catalyzing tRNA acylation using various carboxylic acids as acyl donors and uses thereof. Disclosed is a ribozyme catalyzing tRNA acylation having a structure consisting of the RNA sequence represented by (formula 1):
€ƒ€ƒ€ƒ€ƒ€ƒ€ƒ€ƒ€ƒ(formula 1)€ƒ€ƒ€ƒ€ƒ€ƒP1-Z 1 Z 2 Z 3 Z 4 (N 1 ) 1 (N 1 ) 2 ...(N 1 ) p -P2-(N 2 ) 1 (N 2 ) 2 ...(N 2 ) q Y 1 Y 2 Y 3 (N 3 ) 1 (N 3 ) 2 N 4 GG N
wherein (N 1 ) 1 -(N 1 ) p each independently represent any monoribonucleotide of U, C, A and G; p represents 3 or 4; (N 2 ) 1 -(N 2 ) q each independently represent any monoribonucleotide of U, C, A and G; q represents 5 or 6; (N 3 ) 1 -(N 3 ) 2 each independently represent any monoribonucleotide of U, C, A and G; N 4 represents any monoribonucleotide of U, C, A and G; Z 1 -Z 4 each independently represent C or G; Y 1 -Y 3 each independently represent C or G; N represents a monoribonucleotide complementary to A or G; and P1 and P2 represent a domain consisting of any RNA sequence capable of having a stem-loop structure. |
