BIOMIMETIC MEMBRANE AND BIOSENSOR FOR DETECTING NADH USING THE SAME

摘要

PURPOSE: A biosensor for detecting NADH using a biomimetic membrane is provided to selectively and sensitively detect NADH even at low electric potential. CONSTITUTION: A biomimetic membrane contains 0.2-0.8 mM of phosphoethanol amine, 0.4-1.0 mM of cytochrome c, and 0.2-0.8 mM of ubiquinone. The biomimetic membrane further contains cardiolipin. A biosensor for detecting NADH comprises: a screen printing electrode in which Au nanoparticle layer is deposited; an electric conductive polymer layer coated on the Au nanoparticle layer; and the biomimetic membrane on the polymer layer. The biosensor comprises an additional lipid layer formed of cardiolipin on the biomimetic membrane. The electric conductive polymer is a carboxyl group-activated polyterthiopene polymer.