发明名称 |
IMPROVED TYPE MILK-CLOTTING PROTEASE DERIVED FROM A MICROORGANISM |
摘要 |
Discloses a protease with milk-clotting activity which comprises an amino acid sequence at least 75% identical to SEQ ID NO: 3, said protease has at least one mutation selected from the group consisting of: (A) replacement of glutamine corresponding to glutamine at position 265 in SEQ ID NO: 3 with an acidic amino acid; and (B) replacement of glutamine at position 266 in SEQ ID NO: 3 with an acidic amino acid. Further discloses the said protease with other selected mutations. Further discloses a method for producing a protease with milk-clotting activity comprising the steps of culturing a transformed cell with the disclosed protease sequence in a culture medium and collecting the protease in the culture medium. The disclosed protease finds application in production of cheese and dairy products.
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申请公布号 |
NZ595078(A) |
申请公布日期 |
2012.07.27 |
申请号 |
NZ20100595078 |
申请日期 |
2010.03.19 |
申请人 |
MEITO SANGYO CO., LTD. |
发明人 |
HARADA, KAZUNORI;KOBAYASHI, HIROYUKI;SUGA, TARO;YAMAGUCHI, HIROYUKI;TSUNODA, AKIRA;KATO, SHIGEAKI |
分类号 |
C12N9/58;A23C19/032;C12N15/81 |
主分类号 |
C12N9/58 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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