PHAGE PHI 29 DNA POLYMERASE CHIMERA

摘要

A DNA polymerase chimera comprising an amino-terminal (N-terminal) region encoding a &phgr;29 type DNA polymerase and a carboxyl-terminal (C-terminal) region comprising at least one HhH domain which are bound by means of a connecting amino acid sequence is disclosed along with and the use thereof for replicating, amplifying or sequencing a template DNA. Also disclosed is a method for replicating, amplifying or sequencing a deoxyribonucleic acid with said DNA polymerase chimera and kits for carrying out said methods.