发明名称 |
CELLS FOR TRANSIENT EXPRESSION AND USES THEREOF |
摘要 |
This invention relates to the transient expression of heterologous polypeptides in mammalian cell lines. Specifically it relates to an expression-enhanced cell line derived from a parent cell line, the expression-enhanced cell line comprising nucleic acid encoding Epstein-Barr Virus Nuclear Antigen 1 or a functional derivative, analogue, or variant thereof; and further comprising: (a) a nucleic acid encoding an exogenous glutamine synthetase; (b) a nucleic acid encoding an endogenous glutamine synthetase, wherein the endogenous glutamine is arranged to have enhanced enzymatic activity and/or enhanced expression relative to the parent cell line under comparable conditions; or (c) both (a) and (b). |
申请公布号 |
EP2478107(A2) |
申请公布日期 |
2012.07.25 |
申请号 |
EP20100771684 |
申请日期 |
2010.09.15 |
申请人 |
MEDIMMUNE LIMITED |
发明人 |
DARAMOLA, OLALEKAN;DEAN, GREGORY;HATTON, DIANE;STEVENSON, JESSICA |
分类号 |
C12N15/85;C12N7/02 |
主分类号 |
C12N15/85 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|