发明名称 |
PRODUCTS AND METHODS FOR ENHANCED TRANSGENE EXPRESSION AND PROCESSING |
摘要 |
<p>Disclosed are methods and eukaryotic host cells for transgene expression. The cells may be treated and/or modified to increase homologous recombination (HR), decrease non homologous end joining (NHEJ) and/or to enhance a HR/NHEJ ratio in said cell. Such cells can be transfected with vectors comprising the transgene, which advantageously integrates into the genome of the cell to form a concatemeric structure which may comprise more than 200 transgene copies. Certain expression enhancing elements such as MARs are advantageously provided to further enhance and/or facilitate transgene expression. Disclosed is also a recombinant eukaryotic host cell, in particular a non-primate host cell, comprising a transgenic sequence encoding a protein and/or a RNA, in particular a primate protein and/or RNA, involved in translocation across the ER membrane and/or secretion across the cytoplasmic membrane.</p> |
申请公布号 |
EP2478108(A2) |
申请公布日期 |
2012.07.25 |
申请号 |
EP20100773969 |
申请日期 |
2010.09.20 |
申请人 |
SELEXIS S.A. |
发明人 |
MERMOD, NICOLAS;GIROD, PIERRE-ALAIN;GRANDJEAN, MELANIE;LE FOURN, VALERIE;CALABRESE, DAVID;REGAMEY, ALEXANDRE |
分类号 |
C12N15/90;C12N15/113 |
主分类号 |
C12N15/90 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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