发明名称 |
Methods of purifying chondroitinase and stable formulations thereof |
摘要 |
An aspect of the present invention relates to stable formulations of chondroitinase and to methods of purifying chondroitinase. The methods of purifying chondroitinase includes the steps of extracting the enzyme from a cell, separating the chondroitinase from the crude cell extract using cation-exchange chromatography, removing impurities through gel filtration chromatography, and removing endotoxin through an anion-exchange membrane to produce a purified chondroitinase. |
申请公布号 |
US8226941(B2) |
申请公布日期 |
2012.07.24 |
申请号 |
US20050568831 |
申请日期 |
2005.05.18 |
申请人 |
CAGGIANO ANTHONY O.;GRUSKIN ELLIOTT A.;SHEPTOVITSKY YELENA G.;KASPERBAUER SARAH;ACORDA THERAPEUTICS, INC. |
发明人 |
CAGGIANO ANTHONY O.;GRUSKIN ELLIOTT A.;SHEPTOVITSKY YELENA G.;KASPERBAUER SARAH |
分类号 |
A61K38/46;A61K38/00;A61K38/47;C07H21/04;C12N9/24;C12N9/88 |
主分类号 |
A61K38/46 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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