发明名称 |
Enzyme Triggered Redox Altering Chemical Elimination (E-Trace) Immunoassay |
摘要 |
Described are methods for detecting a target analyte in a test sample by providing a solid support comprising an electrode comprising: (i) a self-assembled monolayer (SAM), (ii) a covalently attached electroactive active moiety (EAM) comprising a transition metal complex comprising a self-immolative moiety and a peroxide sensitive moiety (PSM), wherein said EAM has a first E0; then contacting the target analyte and said solid support under conditions wherein said target analyte reacts with a peroxide generating enzyme to generate peroxide and said self-immolative moiety is removed such that said EAM has a second E0 test sample; and then detecting said second E0 as an indication of the presence of said target analyte. Also provided are compositions for use in the preceding methods. |
申请公布号 |
US2012181186(A1) |
申请公布日期 |
2012.07.19 |
申请号 |
US201213354200 |
申请日期 |
2012.01.19 |
申请人 |
BERTIN PAUL A.;AHRENS MICHAEL J.;OHMX CORPORATION |
发明人 |
BERTIN PAUL A.;AHRENS MICHAEL J. |
分类号 |
C12Q1/28;C12Q1/48 |
主分类号 |
C12Q1/28 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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