发明名称 |
SEQUENCE-SPECIFIC EXTRACTION AND ANALYSIS OF DNA-BOUND PROTEINS |
摘要 |
An automated protein preparation technology that may use magnetic microparticles to isolate proteins in their native state from specific genomic loci of interest via the chromatin to which they are bound is described. After extraction, the targeted proteins may be purified for downstream analysis by quantitative mass spectrometry or ELISA. The identification of DNA-bound proteins, histones and their post-translational modification is of high scientific and pharmaceutical importance due to the role of DNA-binding proteins in the causation and development of human disease; in particular, cancer. |
申请公布号 |
WO2012064954(A3) |
申请公布日期 |
2012.07.19 |
申请号 |
WO2011US60185 |
申请日期 |
2011.11.10 |
申请人 |
THE TRUSTEES OF PRINCETON UNIVERSITY;DAPPRICH, JOHANNES;GARCIA, BENJAMIN;LEROY, GARY |
发明人 |
DAPPRICH, JOHANNES;GARCIA, BENJAMIN;LEROY, GARY |
分类号 |
G01N33/68;C12Q1/68;G01N33/15;G01N33/53 |
主分类号 |
G01N33/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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