| 摘要 |
In one aspect, a process for the intracellular expression of CRM197 in an E coli host is provided. The process comprises expressing a vector comprising a gene encoding CRM 197 operably linked to a promoter and at least one perfect palindrome operator sequence. In a related aspect, a process for the periplasmic expression of CRM197 in an E coli host is provided, comprising expressing a vector comprising a gene encoding CRM197 and a secretion leader sequence operably linked to a weak promoter system comprising at least one perfect palindrome operator sequence. |
