FLUORESCENT CAMH BIOSENSOR

摘要

The invention relates to a fluorescent CaMh (calmodulin) biosensor characterised by a specific site marker with two fluorophores placed in strategic positions (position 39 and 91) in such a way that, on interaction with the ligand, the necessary conformational changes that modify the microenvironment of the fluorophores covalently bound to the protein, and produce a spectroscopic response associated with the interaction, are produced in the protein. Said response enables the determination not only of CaM antagonist products, but also of the parameters of the protein-ligand interaction.