发明名称 |
Method for enhanced sensitivity in bacteriophage-based diagnostic assays |
摘要 |
A method of determining the presence or absence of a target microorganism in a sample to be tested, the method comprising: (a) combining with the sample an amount of bacteriophage capable of attaching to the target microorganism to create a bacteriophage-exposed sample; (b) providing conditions to the bacteriophage-exposed sample sufficient to allow the bacteriophage to attach to the target microorganism while inhibiting phage replication in a potentially cross-reactive, non-target microorganism; and (c) assaying the bacteriophage-exposed sample to detect the presence or absence of a bacteriophage marker to determine the presence or absence of the target microorganism; wherein the amount of the bacteriophage is between 10% to 70% of the threshold number of bacteriophage that the assay can detect, or between 1×106 pfu/mL and 7×106 pfu/mL. |
申请公布号 |
US8216780(B2) |
申请公布日期 |
2012.07.10 |
申请号 |
US20090476796 |
申请日期 |
2009.06.02 |
申请人 |
SMITH JONATHAN DREW;DREILING BREANNA LEIGH;SMITH BREANNA CHRISTINE;WHEELER JOHN H.;MICROPHAGE (TM) INCORPORATED |
发明人 |
SMITH JONATHAN DREW;DREILING BREANNA LEIGH;SMITH BREANNA CHRISTINE;WHEELER JOHN H. |
分类号 |
C12Q1/70;C12Q1/02 |
主分类号 |
C12Q1/70 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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