发明名称 METHOD FOR INVESTIGATION MITOCHONDRIAL BREATH OF ACINAR CELLS OF PANCREATIC GLAND IN SITU
摘要 A method for investigation mitochondrial breath of acinar cells of pancreatic gland in situ, according to which the suspension of isolated acinus of pancreatic gland is produced, the isolation medium is changed to incubation medium, plasmatic membrane of the cells is permeabilized by non-ionogenic detergent – digitonin, and the permeability level of plasmatic membrane is controlled, and oxygen uptake rate is registered by polarographic method. Sucrose is used as an osmotic component of incubation medium.
申请公布号 UA71234(U) 申请公布日期 2012.07.10
申请号 UA20110014671U 申请日期 2011.12.12
申请人 IVAN FRANKO LVIV NATIONAL UNIVERSITY 发明人 MANKO BOHDAN OLEKSIIOVYCH;MANKO VOLODYMYR VASYLIOVYCH;KLEVETS MYRON YURIIOVYCH
分类号 C12N5/02 主分类号 C12N5/02
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