A METHOD OF EXTRACTING COLLAGEN BY ULTRASONICATION

摘要

PURPOSE: An extraction method of collagen using ultrasonic waves is provided to minimize the usage of chemical substances for maximizing the yield of the collagen from the fish skin. CONSTITUTION: An extraction method of collagen using ultrasonic waves comprises the following steps: processing the fish skin with the ultrasonic waves in an acid solution to obtain a collagen extract; separating the collagen and the ultrasonic wave-processed fish skin; and repeating the previous steps to obtain the collagen. The acid solution is 0.01-0.5M acetic acid. The ultrasonic wave-processing step is performed in the frequency of 20kHz for 0.1-24 hours at 0-10 deg C.