发明名称 |
Methods for stratification and storage of somatic embryos |
摘要 |
In one aspect, a method is provided for producing stratified cotyledonary conifer somatic embryos. The method comprises (a) incubating a culture comprising immature conifer somatic embryos in a culture vessel comprising a development medium having an osmolality in the range of from 300 mM/Kg to 450 mM/Kg at a temperature of from 22° C. to 25° C. for a first incubation period sufficient in length for at least a portion of the embryos to reach anatomical maturity; and (b) subjecting the embryos in the culture vessel in accordance with step (a) to a temperature of from 0° C. to 10° C. for a second incubation period of at least one week to produce stratified cotyledonary somatic embryos. |
申请公布号 |
US8216840(B2) |
申请公布日期 |
2012.07.10 |
申请号 |
US20080235460 |
申请日期 |
2008.09.22 |
申请人 |
JAMRUSZKA AMY M;WEYERHAUSER NR COMPANY |
发明人 |
JAMRUSZKA AMY M |
分类号 |
C12N5/00;A01H5/00;A01H9/00;A01H11/00;C12N5/02 |
主分类号 |
C12N5/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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