| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a method of validating microbial preparations using a ribozyme that is specific for a microorganism being prepared. <P>SOLUTION: A method of detecting the presence of an adventitious agent in a composition comprising a reovirus includes: (a) providing a population of indicator cells that expresses a ribozyme, wherein the ribozyme is capable of specifically cleaving the genome of the reovirus; (b) contacting the indicator cells with the composition under conditions that allow for cleavage of the genome of the reovirus by the ribozyme; and (c) determining the effect of the composition on the indicator cells, wherein any pathogenic effect indicates the presence of the adventitious agent in the composition in addition to the reovirus. <P>COPYRIGHT: (C)2012,JPO&INPIT |
