发明名称 |
High Density Sequence Detection Methods |
摘要 |
A method for performing PCR on a liquid sample comprising a plurality of polynucleotide targets, wherein each polynucleotide target is present at very low concentration within the sample. The method comprises applying PCR reactants to the surface of a substrate to produce a plurality of reaction spots on the surface of the substrate; loading the liquid sample and a PCR reagent mixture onto the reaction spots; forming a sealed reaction chamber, having a volume of less than about 20 nanoliters, over each of the reaction spots; and amplifying the sample. |
申请公布号 |
US2012172252(A1) |
申请公布日期 |
2012.07.05 |
申请号 |
US201213420998 |
申请日期 |
2012.03.15 |
申请人 |
WOUDENBERG TIMOTHY M.;JONES ROBERT C.;LIVAK KENNETH J.;LIFE TECHNOLOGIES CORPORATION |
发明人 |
WOUDENBERG TIMOTHY M.;JONES ROBERT C.;LIVAK KENNETH J. |
分类号 |
C40B30/04;C12N;C12P19/34;C12Q1/68;C40B40/06;G01N |
主分类号 |
C40B30/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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