发明名称 |
PRODUCTION OF HIGH MANNOSE GLYCOSYLATED PROTEINS STORED IN THE PLASTID OF MICROALGAE |
摘要 |
The present invention concerns a transformed microalga producing a protein harboring a "high mannose" pattern of glycosylation in the plastid of said transformed microalga, wherein 1) said transformed microalga has a Chloroplast Endoplasmic Reticulum (CER); 2) said microalga has been transformed with a nucleic acid sequence operatively linked to a promoter, said nucleic acid sequence encoding an amino acid sequence comprising (i) an amino-terminal bipartite topogenic signal (BTS) sequence composed of at least a signal peptide followed by a transit peptide; and (ii) The sequence of said protein, 3) the xylosyltransferases and fucosyltransferases of said microalga have not been inactivated; 4) the N-acetylglycosyltransferase I of said microalga has not been inactivated, preferably the N-acetylglycosyltranferases II, III, IV, V and VI, mannosidase II and glycosyltransferases of said microalga have not been inactivated. |
申请公布号 |
WO2012089342(A1) |
申请公布日期 |
2012.07.05 |
申请号 |
WO2011EP06592 |
申请日期 |
2011.12.28 |
申请人 |
ALGENICS;CARLIER, AUDE;MICHEL, REMY;CADORET, JEAN-PAUL;LEJEUNE, ALEXANDRE;DUFOURMANTEL, NATHALIE |
发明人 |
CARLIER, AUDE;MICHEL, REMY;CADORET, JEAN-PAUL;LEJEUNE, ALEXANDRE;DUFOURMANTEL, NATHALIE |
分类号 |
C12N15/82;C12P21/00;C12R1/89 |
主分类号 |
C12N15/82 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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