| 摘要 |
Enzyme substrate of 2-(2'-amidophenyl)-indole compound (I), for detecting peptidase activity, is new. Enzyme substrate of 2-(2'-amidophenyl)-indole compound of formula (I), for detecting peptidase activity, is new. X : S, NX1, O or NX1-CO; R1 : nothing or a substituent comprising Cl, Br, F, I, OH, alkyl, aryl or carboxyl; R2 : nothing or a substituent comprising Cl, O-CH 2-O, O-CH 3, F, diethylenediamine-CH 3, NR3R4, Br, I, OH, alkyl, aryl or carboxyl; R3R4 : H or 1-4C alkyl; X1 : H, C 2H 4Ph, OH alkyl or aryl; and P1 : peptide. Independent claims are included for: (1) a reactional medium comprising the enzyme substrate; (2) a medium for detecting and/or identifying microorganisms, comprising at least the enzyme substrate; and (3) a method for detecting peptidase activity in microorganisms and for differentiating bacteria and their appearance in Gram positive or Gram-negative bacteria, comprising disposing the detection and/or identification medium, seeding the medium with a biological sample to be tested, incubating, and exposing the presence of at least a peptidase activity. [Image]. |
