Method for determining coagulation inhibitors

摘要

Determining an inhibitor of a coagulation factor in a sample, comprises (a) providing and incubating a reaction mix comprising e.g. (i) an aliquot of the sample, (ii) a defined amount of a coagulation factor, (iii) at least one ligand which binds to the active site of the coagulation factor but is not cleaved by the latter at a peptide bond, and (b) measuring the signal of the signal-producing system, where the amplitude of the signal is inversely proportional to the inhibitor concentration in the sample. Determining an inhibitor of a coagulation factor in a sample, comprises (a) providing and incubating a reaction mix comprising (i) an aliquot of the sample, (ii) a defined amount of a coagulation factor, (iii) at least one ligand which binds to the active site of the coagulation factor but is not cleaved by the latter at a peptide bond, (iv) a first and a second component of a signal-producing system which act together in such a way that a detectable signal is generated when the first and second components of the signal-producing system are brought into close proximity to each other, where the coagulation factor is associated with the first component of the signal-producing system or will be associated in that location during incubation; and the ligand is associated with the second component of the signal-producing system or will be associated in that location during incubation, and (b) measuring the signal of the signal-producing system, where the amplitude of the signal is inversely proportional to the inhibitor concentration in the sample. Independent claims are included for: a test kit for carrying out the above method, comprising the following separate components: a first reagent comprising a defined amount of a coagulation factor, a second reagent comprising at least one ligand which binds to the active site of the coagulation factor but is not cleaved by the latter at a peptide bond and a first component of a signal-producing system, which component is associable with the coagulation factor from the first reagent, a third reagent comprising a first component, a second component of the signal-producing system, which component is associable with the ligand from the second reagent and at least one ligand, and a fourth reagent comprising a second component of a signal-producing system, which component is associable with the ligand from the second reagent.