发明名称 |
Comparative genomic hybridization |
摘要 |
The present invention relates to a method of comparing at least one chromosome or part thereof from a cell with a first karyotype with the corresponding chromosome or part thereof from a cell with a second karyotype. The method includes the steps of: (a) amplifying DNA from an isolated chromosome or part of an isolated chromosome; (b) attaching the amplified DNA to a solid substrate; (c) amplifying DNA from one or more cells with a first karyotype and amplifying DNA from one or more cells with a second karyotype; (d) labelling the amplified DNA from the one or more cells with a first karyotype with a first label, and labelling the amplified DNA from the one or more cells with a second karyotype with a second label, wherein the first and second labels are detectably different; (e) hybridizing the amplified and labelled DNA from the one or more cells with a first karyotype to the amplified DNA attached to the solid substrate, and hybridizing the amplified and labelled DNA from the one or more cells with a second karyotype to the amplified DNA attached to the solid substrate; and (f) comparing the relative amount of first and second labels hybridized to the amplified DNA attached to the solid substrate. |
申请公布号 |
US8211642(B2) |
申请公布日期 |
2012.07.03 |
申请号 |
US20040551150 |
申请日期 |
2004.04.02 |
申请人 |
HUSSEY NICOLE DOMINIQUE;HU DONG GUI;ADELAIDE RESEARCH & INNOVATION PTY LTD |
发明人 |
HUSSEY NICOLE DOMINIQUE;HU DONG GUI |
分类号 |
C12Q1/68;C07H21/04;C12P19/34;G01N33/52;G01N33/574 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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