| 摘要 |
PURPOSE: Fermented deer antler extract having improved content of specific free amino acids, and a preparation method thereof are provided to add enzymes to a deer antler extract for fermenting and improving a body absorption rate. CONSTITUTION: A preparation method of fermented deer antler extract having improved content of specific free amino acids comprises the following steps: adding water to deer antler slices having the thickness of 2-5mm, and extracting the deer antler slices 3 times at 100 deg C for 24 hours to obtain a deer antler extract; sterilizing the obtained deer antler extract at 85 deg C for 60 minutes, and decompress-concentrating the extract; adding 0.2% of bacillus subtilis derived proteinase into the concentrate, fermenting the mixture at 40 deg C for 3 hours, and adding 0.1% of bacillus subtilis derived proteinase into the fermented mixture for fermenting the mixture again at 40 deg C for 3 hours to obtain a first fermented material; adding 0.2% of aspergillus oryzae derived amino peptidase into the first fermented material for fermenting at 50 deg C for 3 hours, and adding 0.1% of aspergillus oryzae derived amino peptidase into the mixture for fermenting the mixture again at 50 deg C for 3 hours to obtain the fermented deer antler liquid; sterilizing and decompress-concentrating the fermented deer antler liquid; and adding tyrosine, arginine, alanine, and other compounds to the fermented deer antler liquid.
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