PRIMER SET FOR GIBBERELLA ZEAE PRODUCING DEOXYNIVALENOL AND KIT USING THE SAME

摘要

PURPOSE: A primer pair for detecting deoxynivalenol(DON)-producing Gibberella zeae and a kit using the same are provided to quickly and accurately diagnose DON. CONSTITUTION: A primer pair for detecting DON-producing Gibberella zeae is denoted by sequence numbers 1 and 2. The Gibberella zeae is Fusarium graminearum. A kit for detecting the Gibberella zeae contains the primer pair. A method for detecting the Gibberella zeae using the primer pair comprises: a step of isolating RNA from a sample of Oryza sativa, barley, and wheat; a step of amplifying a target sequence by RT-PCR; and a step of detecting a PCR product.