发明名称 |
PROCESS FOR PREAPRING THE NANOIMMUNOSORBENT SILICON DIOXIDE-AMINOPROPYLTRIETHOXYSILANE-GLUTARALDEHYDE-BOVINE SERUM ALBUMIN-3,6-DICHLORO-2-METHOXYBENZOIC ACID TO BE USED IN THE ELISA TECHNIQUE FOR DOSING THE 3,6-DICHLORO-2-METHOXYBENZOIC ACID PESTICIDE |
摘要 |
<p>The invention relates to a process for preparing a nanoimmunosorbent silicon dioxide-aminopropyltriethoxysilane-glutaraldehyde-bovine serum albumin-3,6-dichloro-methoxybenzoic acid to be used in the ELISA technique for dosing the 3,6-dichloro-2-methoxybenzoic acid by treating the SiOnanoparticles with 10% HNOsolution, with incubation with the silicone derivative at 70°C, activation of nanoparticles isolated and washed with 0.1% glutaraldehyde, suspension of nanoparticles isolated and washed in phosphate buffer, admixing of the separately obtained immunogenic conjugate bovine serum albumin-3,5-dichloro-2-methoxybenzoic acid while stirring for 2 hours, after which the immunosorbent as nanoparticles is separated by centrifugation, followed by washing with phosphate buffer and a second centrifugation.</p> |
申请公布号 |
RO127570(A2) |
申请公布日期 |
2012.06.29 |
申请号 |
RO20100001271 |
申请日期 |
2010.12.02 |
申请人 |
INSTITUTUL NA&TCEDIL,IONAL DE CERCETARE-DEZVOLTARE PENTRU FIZIC&ABREVE,&SCEDIL,I INGINERIE NUCLEAR&ABREVE, "HORIA HULUBEI" |
发明人 |
DOROBAN&TCEDIL,U IOAN;NEAGU LIVIA |
分类号 |
G01N33/53;G01N33/68 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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