METHOD FOR EXTRACTING HUMAN SERUM ALBUMIN FROM TRANSGENIC RICE GRAIN

摘要

The present invention provides a method for extracting human serum albumin from transgenic rice, which comprises the steps of: 1) grinding the dehusked rice grain, then mixing the milled rice grain with an extracting buffer and extracting with stirring to obtain mixture I; 2) adjusting pH of the mixture I to 4.0-4.5 and precipitating it for 1-12 hours to obtain mixture II; 3) filtrating the mixture II and collecting the filtrate to obtain a solution containing high concentration of human serum albumin. The method provides a basis for the further purification of rHSA.