发明名称 |
BIOASSAY METHOD FOR DETECTING PHYSIOLOGICALLY ACTIVE SUBSTANCE |
摘要 |
Provided are a procedurally simple and safe method and kit for measuring the concentration in a biological sample of a physiologically active substance which binds to a receptor that causes a change in intracellular cAMP concentration. By providing a composition including genetically-modified cells which show co-expression of a receptor that causes a change in intracellular cAMP concentration, a cyclic nucleotide-responsive calcium channel, and aequorin luminescent protein, it is possible to measure how much of a physiologically active substance which binds to said receptor is included in a biological sample. |
申请公布号 |
WO2012086756(A1) |
申请公布日期 |
2012.06.28 |
申请号 |
WO2011JP79806 |
申请日期 |
2011.12.22 |
申请人 |
OTSUKA PHARMACEUTICAL CO., LTD.;ARAKI, NAOHIRO;IIDA, MITSURU;MACHIDA, KIYOTAKA |
发明人 |
ARAKI, NAOHIRO;IIDA, MITSURU;MACHIDA, KIYOTAKA |
分类号 |
C12N15/09;C12N5/10;C12Q1/02;C12Q1/04 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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