GENE ENCODING COLD-ACTIVE CELLULASE AND USES THEREOF

摘要

PURPOSE: A cellulase protein isolated from Bacillus sp. DK42 is provided to maintain activity at low temperature and to save energy for producing bio energy from lignocellulosic biomass. CONSTITUTION: A cellulase protein has an amino acid of sequence number 2 and is derived from Bacilus sp. DK42. The protein has 45-60% maximum enzyme activity at 4-15 degree Celsius. A gene encoding the cellulase protein has a base sequence of sequence number 1. A recombinant vector contains a gene encoding the cellulaase protein. A transformed E.coli is prepared by transforming E.coli with the recombinant vector.