发明名称 |
AFFINITY CHROMATOGRAPHY MATRIX |
摘要 |
<p>The present invention relates to a method of separating one or more immunoglobulin containing proteins from a liquid. The method includes first contacting the liquid with a separation matrix comprising ligands immobilised to a support; allowing the immunoglobulin containing proteins to adsorb to the matrix by interaction with the ligands; followed by an optional step of washing the matrix containing the immunoglobulin containing proteins adsorbed thereon; and recovering said immunoglobulin containing proteins by contacting the matrix with an eluent which releases the proteins. The method improves upon previous separation methods in that each of the ligands comprises one or more of a protein A domain (E, D, A, B, C), or protein Z, or a functional variant thereof, with at least one of the monomers having a substitution of the Asparagineor Histidine at the position corresponding to H18 of B domain of Protein A or Protein Z, and wherein the ligand provides an increase in elution pH compared to non-substituted ligand.</p> |
申请公布号 |
WO2012087231(A1) |
申请公布日期 |
2012.06.28 |
申请号 |
WO2011SE51537 |
申请日期 |
2011.12.19 |
申请人 |
GE HEALTHCARE BIO-SCIENCES AB;BJOERKMAN, TOMAS;NOREN, BJORN;RODRIGO, GUSTAV;VASIC, JELENA;AABERG, PER-MIKAEL |
发明人 |
BJOERKMAN, TOMAS;NOREN, BJORN;RODRIGO, GUSTAV;VASIC, JELENA;AABERG, PER-MIKAEL |
分类号 |
B01D15/38;B01J20/281;C07K1/22;C07K14/31;C12N15/03 |
主分类号 |
B01D15/38 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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