Stabilisation of enzymes with stable coenzymes

摘要

Stabilization of an enzyme, comprises storing the enzyme in the presence of a stabilized coenzyme or natural coenzyme, where the enzyme is: an alcohol-dehydrogenase (EC 1.1.1.2), a glucose-6-phosphate dehydrogenase (EC 1.1.1.49) or a diaphorase (EC 1.6.99.2) (when stabilized coenzyme is present), or e.g. an alcohol-dehydrogenase (EC 1.1.1.1; EC 1.1.1.2), a L-amino acid-dehydrogenase (EC 1.4.1.5), a glucose-dehydrogenase (EC 1.1.1.47) or a diaphorase (EC 1.6.99.2) (when natural coenzyme is present). Stabilization of an enzyme, comprises storing the enzyme in the presence of a stabilized coenzyme or natural coenzyme, where the enzyme is: an alcohol-dehydrogenase (EC 1.1.1.2), a glucose-6-phosphate dehydrogenase (EC 1.1.1.49) or a diaphorase (EC 1.6.99.2) (when stabilized coenzyme is present), or an alcohol-dehydrogenase (EC 1.1.1.1; EC 1.1.1.2), a L-amino acid-dehydrogenase (EC 1.4.1.5), a glucose-dehydrogenase (EC 1.1.1.47), a glucose-6-phosphate dehydrogenase (EC 1.1.1.49), a glycerin-dehydrogenase (EC 1.1.1.6), a 3-hydroxybutyrate-dehydrogenase (EC 1.1.1.30), a lactate-dehydrogenase (EC 1.1.1.27; EC 1.1.1.28), a malate dehydrogenase (EC 1.1.1.37) or a diaphorase (EC 1.6.99.2) (when natural coenzyme is present). An independent claim is included for an enzyme stabilized with the stabilized coenzyme, where: the stabilization takes place in the presence of light with a wavelength of >= 300 nm, optionally with high humidity and/or in the absence of drying agent, and the enzyme exhibits a decrease in its activity of less than 50, preferably 20% with respect to the initial value of the enzyme activity.