| 摘要 |
Provided herein are compositions and methods for genetic engineering of cyanobacteria to produce ethanol. In one aspect, the present invention provides a polynucleotide construct comprising a copper ion inductive promoter and a sequence encoding a pyruvate decarboxylase (pdc) enzyme. In another aspect, the present invention provides a genetically engineered cyanobacterium comprising the polynucleotide construct of the invention, wherein the cyanobacterium is capable of producing ethanol after a period of fermentation. In yet another aspect, the present invention discloses a method of producing ethanol by genetically modifying cyanobacteria using the polynucleotide construct of the invention. |
