发明名称 |
Compositions and methods for detecting Borrelia afzelii |
摘要 |
Disclosed are oligonucleotides useful in methods for determining whether a sample contains Borrelia afzelii, a spirochete which is a causative agent of Lyme disease in humans. These oligonucleotides, which have nucleotide sequences derived from a coding segment of the gene encoding the p24 gene for the outer surface protein of Borrelia afzelii, are useful as forward and reverse primers for a polymerase chain reaction using nucleic acids from a biological sample as templates, and as probes for detecting any resultant amplicon. Detection of an amplicon indicates the sample contains Borrelia afzelii. Real-time PCR and detection using florescence resonance energy transfer is disclosed. |
申请公布号 |
US8206930(B2) |
申请公布日期 |
2012.06.26 |
申请号 |
US201113199093 |
申请日期 |
2011.08.18 |
申请人 |
FEOLA MELANIE;ADELSON MARTIN;MORDECHAI ELI;NOVAK LISA;MEDICAL DIAGNOSTICS LABORATORIES, LLC |
发明人 |
FEOLA MELANIE;ADELSON MARTIN;MORDECHAI ELI;NOVAK LISA |
分类号 |
C12Q1/68;C07H21/02;C07H21/04;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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