PRIMERS FOR DIAGNOSIS OF APPLE MOSAIC VIRUS(APMV) INFECTION AND METHOD FOR DIAGNOSIS USING REAL-TIME PCR

摘要

PURPOSE: A primer for diagnosing apple mosaic virus(ApMV) infection using SYBR Green I is provided to accurately diagnose the infection and to enhance detection efficiency. CONSTITUTION: A primer set for diagnosing apple mosaic virus(ApMV) infection contains a primer of sequence number 1 and a primer of sequence number 2. A method for real-time detection method for ApMV comprises: a step of performing real-time PCR using sample DNA or partial DNA using a marker and primer set; and a step of analyzing curve of the real-time PCR products. The marker is SYBR green I.