发明名称 |
Methods and compositions for analyzing AHASL genes |
摘要 |
The invention relates to methods and compositions for analyzing plant acetohydroxy acid synthase large subunit (AHASL) genes. In particular, the invention relates to methods for the detection of wild-type AHASL alleles and mutant AHASL alleles that encode imidazolinone-tolerant AHASL proteins. The methods involve the use of PCR amplification and novel compositions comprising allele-specific and gene-specific primers to detect the presence of mutant and/or wild-type alleles present at the individual AHASL genes of a plant. Specifically, the methods and compositions are useful for analyzing the three AHASL genes of Triticum aestivum and the two AHASL genes of Triticum turgidum ssp. durum.
|
申请公布号 |
US8206906(B2) |
申请公布日期 |
2012.06.26 |
申请号 |
US20080205246 |
申请日期 |
2008.09.05 |
申请人 |
ZHAO CHENGYAN;ASCENZI ROBERT;SINGH BIJAY K.;BASF SE |
发明人 |
ZHAO CHENGYAN;ASCENZI ROBERT;SINGH BIJAY K. |
分类号 |
C12Q1/68;C07H21/02;C07H21/04;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|