发明名称 Pufa polyketide synthase systems and uses thereof
摘要 The present invention relates to a method to produce an isolated nucleic acid molecule selected from the group consisting of: (a) a nucleic acid molecule comprising a nucleic acid sequence encoding 5 an amino acid sequence selected from the group consisting of SEQ ID NO:4 and an amino acid sequence that is at least about 85% identical to SEQ ID NO:4 over the full-length of SEQ ID NO:4, wherein said amino acid sequence has at least one biological activity selected from the group consisting of: p-keto acyl-ACP synthase (KS) 10 activity, chain length factor (CLF) activity, acyl transferase (AT) activity, and enoyl ACP-reductase (ER) activity; (b) a nucleic acid molecule comprising a nucleic acid sequence encoding an amino acid sequence of SEQ ID NO:20; (c) a nucleic acid molecule comprising a nucleic acid sequence encoding 15 an amino acid sequence that is at least about 60% identical to SEQ ID NO:20, and has KS activity; (d) a nucleic acid molecule consisting essentially of a nucleic acid sequence encoding an amino acid sequence selected from the group consisting of: SEQ ID NO:22, SEQ ID NO:24, SEQ ID NO:26, SEQ ID 20 NO:28, SEQ ID NO:30, and SEQ ID NO:32; and (e) a nucleic acid molecule consisting essentially of a nucleic acid sequence encoding an amino acid sequence that is at least about 60% identical to: (i) SEQ ID NO:22, and has CLF activity; 25 (ii) SEQ ID NO:24, and has AT activity; (iii) SEQ ID NO:26 or SEQ ID NO:32, and has ER activity; or (iv) SEQ ID NO:28 or SEQ ID NO:30, and has FabA-like P-hydroxy acyl-ACP dehydrase (DH) activity.
申请公布号 AU2008249168(B2) 申请公布日期 2012.06.21
申请号 AU20080249168 申请日期 2008.11.21
申请人 MARTEK BIOSCIENCES CORPORATION 发明人 KUNER, JERRY M.;BARCLAY, WILLIAM R.;METZ, JAMES G.;FLATT, JAMES H.
分类号 A01H5/00;A61K48/00;A61P1/04;A61P1/16;A61P3/06;A61P9/10;A61P11/00;A61P15/06;A61P19/02;A61P19/10;A61P25/08;A61P25/24;A61P25/28;A61P29/00;A61P31/04;A61P35/00;A61P37/02;A61P43/00;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N15/09;C12N15/52;C12N15/82;C12P1/02;C12P7/62;C12P7/64;C12Q1/02;C12Q1/68;C12R1/645 主分类号 A01H5/00
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