METHOD FOR CONSTRUCTING DNA SEQUENCING LIBRARY AND USE THEREOF

摘要

Provided are two reagents, a method for constructing a DNA sequencing library, a DNA sequencing library, a method for determining the sequence information in a DNA sample and a kit for constructing a DNA sequencing library, wherein one reagent comprises 10 mM to 1000 mM of a buffer salt, 10 mM to 100 mM of a soluble salt, 10 mM to 300 mM of MgCl2, 1 mM to 50 mM of dithiothreitol, 1 mM to 10 mM of dATP and 1 U/ml to 40 U/ml of klenow fragment, with the pH of the reagent being 7.6 to 7.9, and the other reagent comprises 10 mM to 200 mM of a buffer salt, 1 mM to 100 mM of dithiothreitol, 10 mM to 400 mM of MgCl2, 1 mM to 40 mM of ATP and 1 U/ml to 200 U/ml of DNA ligase, with the pH of the reagent being 7.6 to 7.9.