| 摘要 |
FIELD: physics. ^ SUBSTANCE: in method, laser radiation pulses are transmitted through a collimator with a smaller beam divergence angle, which ensures a constant illumination area, wherein at the first step for measuring the level of fluorescence F0, pre-attenuated laser pulses with duration of 10 mcs and frequency 20 kHz are transmitted, said pulses illuminating the plant with luminous density of not more than 1 mcmol/m2s (ëñ240 mW/m2), without activating photosynthesis in said plant, and the received fluorescence radiation, without attenuation, undergoes selection; after a few second, at the second step, non-attenuated laser pulses with duration of 1 s with luminous density of over 2000 mcmol/m2s (>480 W/m2) are transmitted for measuring the level of fluorescence Fm, and the received fluorescence radiation is attenuated before spectral selection to the level of picking up separate photoelectric current pulses. Selective signals obtained at the first and second steps are picked up in photon-counting mode, and maximum quantum output of primary charge separation in the FSP photosystem is determined based on fluorescence levels F0 and Fm measured in the two steps at wavelength 685 nm. ^ EFFECT: determining functional state of the photosynthesis mechanism of plants. ^ 1 dwg |
