发明名称 |
SINGLE PROBE, MULTIPLE TEMPERATURE, NUCLEIC ACID DETECTION METHODS, KITS, AND COMPOSITIONS |
摘要 |
Provided herein are methods, kits, and compositions related to nucleic acid detection assays that allow discrimination of multiple target sequences with a single probe. In particular, provided herein are methods kits, and compositions that include single-probe target sequence discrimination where different target amplicons may have identical probe hybridization sequences by employing multiple temperature end-point signal probe detection. Also provided herein are methods, kits, and compositions for distinguishing between two or more target amplicons using multiple-temperature end-point probe detection. In certain embodiments, asymmetric PCR amplification methods are employed (e.g., LATE-PCR amplification). |
申请公布号 |
EP2464737(A1) |
申请公布日期 |
2012.06.20 |
申请号 |
EP20100808708 |
申请日期 |
2010.08.11 |
申请人 |
BRANDEIS UNIVERSITY |
发明人 |
REIS, ARTHUR, H., JR.;WANGH, LAWRENCE, J.;PIERCE, KENNETH |
分类号 |
C12P19/34;C12Q1/68 |
主分类号 |
C12P19/34 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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