METHOD FOR PRODUCING IMMORTALISED HUMAN CELL, STABLE TRANSFECTED IMMORTALISED HUMAN CELL, METHOD FOR RECOMBINANT PRODUCTION OF TARGET HUMAN PROTEIN, APPLICATION OF TRANSFECTION VECTOR

摘要

FIELD: medicine. ^ SUBSTANCE: method for producing an immortalised human cell involves transfection of an immortalised human host cell in a serum-free medium with using a transfection vector containing a gene coding a target human protein, a promoter and a bovine growth hormone polyadenilation (polyA) signal wherein said promoter and the polyA signal are binded with 5'- and 3'-terminus of the gene coding a target human protein respectively, and an origin of replication. Said transfection vector additionally bears at least one genetic selection marker. Stable transfected cells are selected. ^ EFFECT: invention allows producing the stable immortalised transfected human cells for preparing recombinant human proteins. ^ 16 cl, 16 dwg, 1 tbl, 5 ex