| 摘要 |
A method is provided for introducing a GAG binding site into a protein comprising the steps:
- identifying a region in a protein which is not essential for structure maintenance
- introducing at least one basic amino acid into said site and/or deleting at least one bulky and/or acidic amino acid in said site,
whereby said GAG binding site has a GAG binding affinity of Kd ‰¤ 10µM, preferably ‰¤ 1µM, still preferred ‰¤ 0.1µM, as well as modified GAG binding proteins. |
