MICROSPORE TRANSFORMATION METHODOLOGY BY MACROINJECTION

摘要

A method for introducing a cargo molecule into a microspore of a plant involves macroinjecting a bud of a donor plant in vivo with a complex comprising a nanocarrier and the cargo molecule. The method is particularly useful in a method of transforming plant cells by macroinjection of buds using a nanocarrier:nucleic acid complex. The method preferably involves transformation of microspores in vivo by macroinjection of donor plant buds with a nanocarrier:nucleic acid complex prior to culturing isolated microspores (immature pollen), and subsequent generation of a doubled-haploid homozygous transgenic plant from the transformed microspores.